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目的建立一种石墨炉原子吸收光谱法快速检测全血中铊的方法。方法血样用基体改进剂稀释后,加入100μl硝酸脱去蛋白,离心分离后取上清液测定血中铊。结果在优化条件下,采用1 g/L Ni(NO3)2-0.2%TritonX-100作为混合基体改进剂,有效消除了基体干扰。用工作曲线法定量,方法线性范围0μg/L~50μg/L(r=0.9999),当进样量20μl时,方法最低检出限为0.64μg/L。在加标水平为10μg/L、20μg/L、40μg/L时,加标平均回收率为97.8%~107.3%,相对标准偏差为1.0%~6.8%。结论血样经酸脱蛋白后可直接用于石墨炉原子吸收光谱法测定,方法简便快速,准确度和精密度好,可以满足急慢性铊中毒者血中铊含量的快速检测,为临床诊断治疗提供参考依据。
Objective To establish a rapid method for the determination of thallium in whole blood by graphite furnace atomic absorption spectrometry. Methods Blood samples were diluted with matrix modifier and 100 μl of nitric acid was added to remove the protein. After centrifugation, the supernatant was taken to measure thallium in the blood. Results Under optimized conditions, 1 g / L Ni (NO3) 2-0.2% TritonX-100 was used as a mixed matrix modifier to effectively eliminate matrix interference. The working curve method was used for the quantification. The linear range was 0μg / L ~ 50μg / L (r = 0.9999). When the injection volume was 20μl, the detection limit was 0.64μg / L. The spiked recoveries were 97.8% -107.3% with relative standard deviations of 1.0% -6.8% at the spiked levels of 10μg / L, 20μg / L and 40μg / L. Conclusion The blood samples can be directly deproteinized by graphite furnace atomic absorption spectrometry, the method is simple, rapid, accurate and precise, which can meet the rapid detection of thallium in blood of patients with acute and chronic thallium poisoning, and provide the clinical diagnosis and treatment Reference.