论文部分内容阅读
目的：研究人参皂苷Rh2抗心肌缺血作用及其机制。方法40只Wistar大鼠随机分为5组：对照组(Con)，异丙肾上腺素组(Iso)，人参皂苷Rh2（10、20、40 mg/kg）组。各组大鼠灌胃给予相应药物，除对照组外其余各组大鼠皮下注射盐酸异丙肾上腺素(20 mg/kg)制备心肌缺血模型。末次注射异丙肾上腺素2 h后水合氯醛麻醉大鼠，取血检测血清肌钙蛋白T（TnT）含量、肌酸激酶(CK-MB)和乳酸脱氢酶（LDH）活力，取大鼠左心室制备组织匀浆，检测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力（T-AOC）。结果与Con组相比，Iso组血清TnT含量、CK-MB活力、LDH活力明显增高。与Iso组比较，人参皂苷Rh2（10、20、40 mg/kg）可显著降低血清TnT含量、CK-MB活力、LDH活力；与Con组比较，Iso组大鼠心肌组织MDA含量增高，而SOD活性、GSH-Px活性及T-AOC降低；与Iso组相比，人参皂苷Rh2（10、20、40 mg/kg）能够显著降低心肌组织MDA含量，同时提高SOD活性、GSH-Px活性及T-AOC。结论人参皂苷Rh2具有抗心肌缺血作用，其作用机制与抗氧化有关。“,”Objective] This study aims to investigate the effects of ginsenoside Rh2 on myocardial injury induced by isoproterenol in rats. [Methods] Wistar rats were treated oral y with ginsenoside Rh2 at the doses of 10, 20 or 40 mg/kg for 7 days. Four days after treatment, al animals, except those in the control group, were subcutaneously injected with isoproterenol (20 mg/kg) for 3 consecutive days. Two hours after the last isoproterenol injection, the rats were anaesthetized and sacrificed. The content of Troponin T (TnT) and activities of creatine kinase (CK-MB), lactate dehydrogenase (LDH) in serum and the biochemical parameters in heart tissues were assayed. [Results] It showed that ginsenoside Rh2 reduced the content of TnT, CK-MB and LDH activities. Ginsenoside Rh2 inhibited not only the elevation of malondialdehyde content but also the reduction of the activities of superoxide dismutase, glutathione peroxidase and the total antioxidant capacity. [Conclusion] The present findings suggested that ginsenoside Rh2 exerted cardioprotective effects against myocardial ischemic injury and the mechanisms was related to regulating the antioxidant capacity of heart tissues.