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目的:检测Myostatin基因疫苗的免疫效果,观察其对免疫动物的影响,为Myostatin基因疫苗的应用提供实验研究。方法:以Myostatin基因疫苗pVAC-TT-Ms免疫小鼠,ELISA法测定其免疫小鼠血清的抗体滴度,通过全自动生化分析仪检测血清指标。以组织化学染色(HE)染色检测Myo-statin基因疫苗对免疫小鼠骨骼肌的影响。利用Scion Image4.02分析Myostatin基因疫苗对免疫小鼠肌纤维横截面积的影响。结果:基因疫苗Myostatin能够诱导小鼠产生针对Myo-statin的抗血清。与正常对照组比较,基因疫苗pVAC-TT-Ms免疫的动物平均体重增加了9.8%,股四头肌、腓肠肌和胸大肌分别增加了24.1%、10.9%和20.3%。结论:基因疫苗Myostatin能够诱导小鼠产生针对Myostatin的特异性中和抗体。Myostatin基因疫苗免疫的动物体重增加,肌细胞有肥大现象,肌肉质量明显增加。
Objective: To detect the immunogenicity of Myostatin gene vaccine and observe its effect on the immunized animals, and to provide experimental study on the application of Myostatin gene vaccine. Methods: Mice were immunized with Myostatin gene vaccine pVAC-TT-Ms, the antibody titers of the immunized mice were measured by ELISA, and serum indexes were detected by automatic biochemical analyzer. The effect of Myo-statin gene vaccine on skeletal muscle of immunized mice was detected by histochemical staining (HE). The effect of Myostatin gene vaccine on the cross-sectional area of myofibers in immunized mice was analyzed by Scion Image 4.02. Results: The gene vaccine Myostatin was able to induce mice to produce antiserum against Myo-statin. Compared with the normal control group, the average body weight of the animals immunized with the gene vaccine pVAC-TT-Ms increased by 9.8%, and the quadriceps, gastrocnemius and pectoralis major muscles increased by 24.1%, 10.9% and 20.3% respectively. Conclusion: The gene vaccine Myostatin can induce specific neutralizing antibodies against Myostatin in mice. Myostatin gene vaccinated animals increased body weight, muscle cells hypertrophy, muscle mass increased significantly.