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在水稻白叶枯病的测报和研究中,往往需要从发病组织中分离出白叶枯病菌。过去多采用稀释分离法,但操作程序复杂。近年来我们采用保湿分离法。直接从经消毒的病叶切口处挑出溢出的菌脓,于斜面培养基上进行培养。这种方法,十分简便,成功率一般在90％以上,适合基层应用。 保湿分离法的分离步骤是: 1.将牛肉膏蛋白胨培养基经高压蒸汽消毒30—45分钟,倒入消毒试管做成斜面待用。 2.将河沙洗净,用无菌水冲洗数次,装于培养皿或其它器皿内,并准备一个干净茶杯或其它玻罩待用。
In the report and study of rice bacterial leaf blight, it is often necessary to isolate the bacterial leaf blight from the diseased tissue. In the past, more dilution separation method, but the operating procedures are complicated. In recent years, we use moisture separation method. Directly from the disinfection of the diseased leaf incision out of the bacteria pus splashed, slant culture medium. This method is very simple, the success rate is generally above 90%, suitable for grass-roots applications. Moistening separation step is: 1. The beef extract peptone medium by high pressure steam sterilization 30-45 minutes, into the sterile tube made of beveled stand-by. 2. Wash the river sand, rinse it with sterile water several times, install it in a petri dish or other vessel, and prepare a clean teacup or other glass cover for use.