探讨分离视网膜感光细胞的方法。方法 :采用 0 .2 5 %胰酶 (视网膜感光细胞面朝下法 )和 2 .5 %胰酶 + 0 .2 %胶原酶 +0 .4% EDTA的消化液 (视网膜感光细胞面朝上法 )酶分层消化法分离获取纯视网膜感光细胞。结果 :酶分层消化法可分离获取纯视网膜感光细胞。结论 :酶分层消化法是一种可靠、费用廉价的获取纯视网膜感光细胞的新方法 To explore the method of separating retinal photoreceptor cells. Methods: Using 0.5% trypsin (retinal photoreceptor cell facing down method) and 2.5% trypsin + 0.2% collagenase + 0.4% EDTA digestion solution (retinal photoreceptor cell facing up method ) Enzymatic stratified digestion obtained pure retinal photoreceptor cells. Results: Enzymatic stratified digestion method can obtain pure retinal photoreceptor cells. Conclusion: Stratified enzyme digestion method is a reliable and inexpensive new method to obtain pure retinal photoreceptor cells