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目的了解四川省攀枝花市主要食品中单核细胞增生性李斯特菌、大肠杆菌O157、沙门菌、志贺菌、金黄色葡萄球菌、蜡样芽孢杆菌、阪崎肠杆菌等7种重要食源性致病菌的污染状况,为食品安全风险评估和预防食源性疾病提供科学依据。方法 2012年在全市5个区县抽查熟肉制品、果蔬类、烤焙制品、凉拌菜、调味酱、蛋制品、婴幼儿食品、熟制米面制品8类食物样品共366份,依据国标法进行检测,运用API卡和VTEK2 COMPACT全自动细菌鉴定仪对样品进行病原菌鉴定。结果从366份样品中共检出38株食源性致病菌,总检出率为10.4%,其中金黄色葡萄球菌18株,检出率5.4%;蜡样芽胞杆菌18株,检出率15.7%;单核细胞增生性李斯特菌2株,检出率0.9%。8类食品中病原菌阳性率最高的是熟制米面制品(33.3%),其次为肉制品(9%),第三为烤焙制品(3%)。结论 2012年攀枝花地区居民主要消费食品中存在着较为严重的食源性致病菌的污染,其中熟制米面制品是主要的污染食品。
Objective To understand seven important food-borne pathogens such as Listeria monocytogenes, Escherichia coli O157, Salmonella, Shigella, Staphylococcus aureus, Bacillus cereus and Enterobacter sakazakii in Panzhihua City, Sichuan Province. The contamination of pathogens provides a scientific basis for food safety risk assessment and prevention of foodborne diseases. Methods In 2012, 366 samples of 8 types of food including cooked meat, fruit and vegetable, baked goods, salad, seasoning, egg products, baby food and cooked rice noodles were collected in 5 districts and counties in the city. According to the national standard Detection, use of API card and VTEK2 COMPACT automatic bacterial identification instrument for pathogen identification. Results A total of 38 food-borne pathogens were detected from 366 samples, with a total detection rate of 10.4%, of which 18 strains were Staphylococcus aureus, with a detection rate of 5.4%. Bacillus cereus 18 strains with a detection rate of 15.7 %; Listeria monocytogenes 2 strains, the detection rate of 0.9%. The highest positive rates of pathogens in the eight categories were cooked rice products (33.3%) followed by meat products (9%) and baked products (3%). Conclusion In 2012, there were more serious food-borne pathogenic bacteria in the main consumer food products in Panzhihua, of which cooked rice products were the main contaminated food.