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目的观察高湿环境对肝脏异柠檬酸脱氢酶(IDH)与α-酮戊二酸脱氢酶(α-KGDHC)含量的影响。方法32只SD大鼠按随机数字表法分为常温常湿对照组及高湿环境20、30、40 d组,每组8只大鼠。采用人工气候箱模拟高湿环境,条件设置为温度25℃、相对湿度90%~95%RH。实验组大鼠每天置于箱中10 h,其余时间与常温常湿对照组饲养于清洁环境(温度22~26℃,相对湿度40%~60%RH)。动物模型制作完成后,取腹主动脉血及肝组织,酶联免疫法(ELISA)检测肝脏组织IDH及α-KGDHC,比色法检测血清乳酸含量,并行肝组织HE病理切片观察。结果高湿环境20 d组,肝脏IDH、α-KGDHC及血清乳酸含量较常温常湿对照组无显著变化。高湿环境30、40 d组,肝脏IDH含量分别降至常温常湿对照组的75.98%和79.90%(P<0.05),α-KGDHC含量分别降至常温常湿对照组的80.00%和81.01%(P<0.05)。血清乳酸含量较常温常湿对照组分别升高了34.58%和23.33%(P<0.01)。病理切片显示高湿环境40 d大鼠肝脏点状坏死灶增多。结论高湿环境可致大鼠肝脏组织IDH、α-KGDHC含量降低,血乳酸升高,机体有氧代谢功能不足。
Objective To observe the effect of high humidity on the content of hepatic isocitrate dehydrogenase (IDH) and α-ketoglutarate dehydrogenase (α-KGDHC). Methods Twenty-two SD rats were randomly divided into two groups according to random number table: normal temperature and normal humidity control group and high humidity environment 20, 30 and 40 d groups. The artificial climate chamber is used to simulate the high-humidity environment. The conditions are set as follows: the temperature is 25 ° C and the relative humidity is 90% -95% RH. Rats in the experimental group were placed in the box for 10 h everyday. The rest of the rats in the experimental group were kept in a clean environment (temperature 22-26 ℃, relative humidity 40% -60% RH) with the normal temperature and humidity control group. Abdominal aorta blood and liver tissue were obtained after animal model was made. IDH and α-KGDHC in liver tissues were detected by enzyme-linked immunosorbent assay (ELISA), serum lactate content was detected by colorimetric method, and HE pathological sections of liver tissue were observed. Results Compared with normal temperature and normal humidity control group, the content of IDH, α-KGDHC and serum lactate in 20 d group in high-humidity environment did not change significantly. The content of IDH in the 30,40 d group and the liver decreased to 75.98% and 79.90% (P <0.05) of the normal temperature and normal humidity control group respectively, while the content of α-KGDHC decreased to 80.00% and 81.01% respectively in the normal temperature and normal humidity control group (P <0.05). Serum lactate levels were increased by 34.58% and 23.33% (P <0.01) compared with the normal temperature and humidity control group respectively. The pathological sections showed that the liver punctate necrosis increased in 40 days in high humidity environment. Conclusion High-humidity environment can reduce the content of IDH and α-KGDHC, raise the blood lactate, and lack the function of aerobic metabolism.