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40只SD大鼠随机分为假手术组、脑缺血10min组、脑缺血10min再灌流15min组和脑缺血10min再灌流15min+~ωN—硝基左旋精氨酸(L—NNA)组.采用荧光法测定大鼠4个脑区(大脑皮质、海马、纹状体和小脑)NO_2的变化.结果显示:脑缺血10min组,各脑区NO_2的含量明显高于假手术组(P<0.01);脑缺血10min再灌流15min组,各脑区NO_2的含量高于脑缺血10min组(P<0.05或 P<0.01);L—NNA可明显降低脑缺血10min再灌流15min各脑区NO_2的含量(P<0.05或P<0.01).
Forty SD rats were randomly divided into sham operation group, 10 min ischemia group, 15 min ischemia reperfusion 15 min ischemia group and 15 min reperfusion 15 min ischemia + L-NNA group. The changes of NO 2 in four brain regions (cerebral cortex, hippocampus, striatum and cerebellum) of rats were detected by fluorescence spectroscopy.The results showed that the content of NO 2 in cerebral ischemia 10 min group was significantly higher than that of sham operation group (P < 0.01). The content of NO 2 in each brain area was significantly higher than that in ischemia 10 min group (P <0.05 or P <0.01) after 10 min of ischemia and 10 min of reperfusion. L-NNA significantly reduced the brain NO 2 content (P <0.05 or P <0.01).