【摘 要】
：
将产热稳定性过氧化氢酶的重组大肠杆菌培养后菌体破碎得到的粗酶液经热处理、硫酸铵分级沉淀、DEAE-Sephadex A-50离子交换层析、HiPrep 16/10 Phenyl疏水作用层析、Su-Perd
【机 构】
：
江南大学生物工程学院工业生物技术教育部重点实验室
论文部分内容阅读
将产热稳定性过氧化氢酶的重组大肠杆菌培养后菌体破碎得到的粗酶液经热处理、硫酸铵分级沉淀、DEAE-Sephadex A-50离子交换层析、HiPrep 16/10 Phenyl疏水作用层析、Su-Perdex200 HR 10/30凝胶层析提纯后得到电泳纯的酶,比酶活达到15629U/mg.此酶的最适温度为70℃,最适pH7.0,在60℃保温60min酶活力基本不变,在PH3～8的范围内比较稳定.此酶的Km和Vmax 分别为7.75mmol/L和27.8mnol@min-1@mg-1.1mmol/L的Zn2+、Ba2+、Mn2+可使该酶完全失活,KCN、NaN3、Na2S2O4、巯基乙醇对酶活力有抑制作用,50mmol/L的EDTA不影响酶活性.
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