论文部分内容阅读
目的 研究丙型肝炎病毒 (hepatitis C virus,HCV)非结构 3区 (NS3) C末端 HL A- 11限制的细胞毒 T细胞(CTL )表位在 HCV感染中的作用 .方法 血清学方法检测 3例患者的 HL A分型 ,他们均存在 HL A- A11表型 ,以 HL A- 11结合基序为依据 ,预测了 HL A- 11限制的 CTL 表位 . 2例慢性患者 5 a内 3个时间点和 3a内 2个时间点及转阴患者的血清样本 ,用反转录 PCR扩增出 HCV基因片段 ,Sanger法测定核苷酸序列 ,并翻译成蛋白质 .结果 慢性 HCV感染者 2例预测的 HL A- A11限制的 CTL 表位都未发生明显改变 ,而在1例 HCV转阴者存在 1个改变的 HL A- 11限制性 CTL表位(序列为 IIL THPVTK) ,然而经 T细胞增殖实验表明此表位不是 T细胞表位 .结论 在 HCV NS3蛋白 C末端可能不存在与 HCV转归有关的 HL A- A11限制的 CTL 表位
Objective To investigate the role of cytotoxic T lymphocyte (CTL) epitopes restricted by C-terminal HL-11 restricted by nonstructural region 3 (NS3) of hepatitis C virus (HCV) in HCV infection.Methods The serum levels of 3 In patients with HL A genotype, they both had the HL A11 phenotype, predicting the HL A-11 restricted CTL epitope based on the HL A-11 binding motif, 2 of the 2 chronic patients within 5 a Time point and 2 time points in 3a and negative patients serum samples were amplified by reverse transcription PCR gene fragment, Sanger determination of nucleotide sequence and translated into protein.Results 2 cases of chronic HCV infection were predicted Of HL-A11-restricted CTL epitopes did not change significantly, whereas in 1 HCV-negative individuals, there was 1 altered HLA-11 restricted CTL epitope (sequence IIL THPVTK), whereas T cell proliferation Experiments show that this epitope is not a T-cell epitope.Conclusion There may be no HL-A11 restricted CTL epitope associated with HCV outcome at the C-terminus of HCV NS3 protein