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目的探讨NARC-1蛋白在新西兰兔动脉粥样硬化血管壁中的表达。方法选取健康纯种雄性新西兰白兔30只,适应性喂养1周后,随机分为对照组(15只,普通颗粒饲料喂养)和高胆固醇组(15只,高胆固醇组给予含高胆固醇的饲料),分别喂养12周后处死。分析两组动物主动脉内膜粥样硬化病变面积,免疫组化检测检测两组动物主动脉组织NARC-1蛋白的表达。结果高胆固醇组出现了明显的动脉粥样硬化病变,主动脉内膜面粥样硬化病变面积为(27.41±2.12)%,与对照组(0.51±0.20)%相比差异有统计学意义(P<0.05)。免疫组化证实NARC-1蛋白在高胆固醇组动物主动脉内膜壁较对照组组织呈高表达,差异具有统计学意义(P<0.05)。结论 NARC-1/PCSK9可能是影响动脉粥样硬化病变形成与发展的重要因素,为进一步对高胆固醇血症和动脉粥样硬化药物防治的研究奠定了基础。
Objective To investigate the expression of NARC-1 in the arterial wall of atherosclerosis in New Zealand rabbits. Methods Thirty healthy male New Zealand white rabbits were selected and fed for one week after the adaptive feeding. The rats were randomly divided into control group (15 fed with normal pellet) and high cholesterol group (15 fed with high cholesterol group) ), Were sacrificed after 12 weeks of feeding. The area of atherosclerotic lesions in aortic endothelium of the two groups was analyzed. The expression of NARC-1 protein in the aortic tissue of the two groups was detected by immunohistochemistry. Results There was obvious atherosclerotic lesions in the hypercholesterolaemia group and the area of atherosclerotic lesions in the aorta was (27.41 ± 2.12)%, which was significantly different from that in the control group (0.51 ± 0.20)% (P <0.05). Immunohistochemistry showed that NARC-1 protein was highly expressed in the intima of the aorta in hypercholesterolemic group compared with the control group (P <0.05). Conclusion NARC-1 / PCSK9 may play an important role in the formation and development of atherosclerotic lesions, which lays the foundation for further research on the prevention and treatment of hypercholesterolemia and atherosclerosis.