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目的:比较了3种纯化方法对黄连解毒汤中盐酸小檗碱含量的影响,以优选出黄连解毒汤的水提取液的最佳纯化方法。方法:对黄连解毒汤的水提取液分别用醇沉、大孔树脂吸附和吸附澄清剂澄清3种方法纯化,并用HPLC法测定了所得提取物中盐酸小檗碱的含量,以此为指标对3种纯化方法进行了比较研究。结果:大孔树脂吸附法出膏率最低,醇沉法次之,吸附澄清法最高;盐酸小檗碱含量以大孔树脂吸附法最高,醇沉法次之,吸附澄清法最低。结论:大孔树脂吸附法为黄连解毒汤水提取液的最佳纯化方法。
OBJECTIVE: To compare the effects of three purification methods on the content of berberine hydrochloride in Huanglian Jiedu Decoction to optimize the purification method of Huanglian Jiedu Decoction aqueous extract. Methods: The water extracts of Huanglian Jiedu Decoction were purified by alcohol precipitation, macroporous resin adsorption and adsorption clarifying agent, and the content of berberine hydrochloride in the extract was determined by HPLC. Three kinds of purification methods were compared. Results: The adsorption rate of macroporous resin was the lowest, that of alcohol was the second, and that of adsorption and clarification was the highest. The content of berberine hydrochloride was the highest with the macroporous resin adsorption method, followed by the alcohol precipitation method, and the adsorption and clarification method was the lowest. Conclusion: The macroporous resin adsorption method is the best purification method for Huanglian Jiedu Tang water extract.