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作者曾报告使用高效液相色谱法分析大骨节病区面粉甲醇提取物,发现两种非病区面粉中甚少检出的物质。其中一种在25个样品中全部发现,另一种仅见于5个样品。高效液相色谱法不能为未知物定性。作者改用质谱法继续研究。用相同方法处理的重病区面粉中的确存在数种非病区面粉中甚少的物质。用质谱法初步确认为苏糖醇、木糖醇和两种常见的有机酸。报告如下:
The authors have reported the use of HPLC for the analysis of flour methanol extract in KBD and found little detectable material in the two NDF flour. One of them was found entirely in 25 samples and the other only in 5 samples. High performance liquid chromatography can not be identified for unknown substances. The author switched to mass spectrometry to continue research. There is indeed a very small amount of material in several non-wilted flours in heavily affected area flour treated in the same manner. Mass spectrometry initially identified as threitol, xylitol and two common organic acids. The report is as follows: