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目的:探讨出血坏死表型肝细胞癌(HN-HCC)瘤内微血管(MV)特征。方法:收集104例HCC样本,其中HN-HCC 72例,非HN-HCC(NHN-HCC)32例。将每例HCC样本均划分为癌组织中央区、肿瘤中心到肿瘤边缘中间区、肿瘤边缘区3个区域,分别比较两种类型HCC样本对应区域间MV形态特征以及血管生成相关基因BTB/POZ结构域蛋白7(BTBD7)、缺氧诱生因子1α(HIF-1α)、促血管生成素2(Ang-2)的m RNA表达的差异。结果:HCC瘤内MV形态分为肿瘤细胞簇包绕型血管(VETC)、毛细管状血管(CV)和两者混合型(VETC+CV)。两者相应区域间比较,HN-HCC各区域VETC阳性率均高于NHN-HCC(65%vs.29%、76%vs.9%、70%vs.16%),CV阳性率均低于NHN-HCC组(23%vs.55%、3%vs.72%、11%vs.59%),差异均有统计学意义(均P<0.05),但VETC+CV阳性率均无差异(均P>0.05);HN-HCC各区域BTBD7,HIF-1α,Ang-2 m RNA的表达水平均高于NHN-HCC(均P<0.05)。结论:HN-HCC瘤内MV形态构筑紊乱且血管生成相关基因异常表达,可能是导致肿瘤局部缺血缺氧及侵袭转移重要原因。
Objective: To investigate the characteristics of intracranial microvascular (MV) in hemorrhagic necrotic phenotype hepatocellular carcinoma (HN-HCC). Methods: A total of 104 HCC samples were collected, of which 72 were HN-HCC and 32 were non-HN-HCC (NHN-HCC). Each HCC sample was divided into three regions: the central region of cancer, the center of tumor to the edge of tumor and the marginal region of tumor. The morphological characteristics of MV and the BTB / POZ structure of angiogenesis were compared between two types of HCC samples Domain protein 7 (BTBD7), hypoxia-inducible factor 1α (HIF-1α) and angiopoietin 2 (Ang-2). Results: The tumor morphology of HCC was divided into VETC, CV and VETC + CV. The positive rate of VETC in each region of HN-HCC was higher than that in NHN-HCC (65% vs.29%, 76% vs.9%, 70% vs.16%), and the positive rate of CV was lower than NHN-HCC group (23% vs.55%, 3% vs.72%, 11% vs.59%), the differences were statistically significant (all P <0.05), but there was no difference in the positive rate of VETC + (All P> 0.05). The expression levels of BTBD7, HIF-1α and Ang-2 mRNA in HN-HCC were higher than those in NHN-HCC (all P <0.05). Conclusion: The morphological changes of MV in HN-HCC and the abnormal expression of angiogenesis-related genes may be the important reasons leading to ischemic hypoxia and invasion and metastasis of tumor.