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目的建立RP-HPLC测定血浆中阿苯达唑及其代谢物阿苯达唑亚砜、阿苯达唑砜浓度的方法。方法在改进文献报道的基础上,采用C2柱固相萃取法处理血浆,建立了精确、灵敏、重现性和专一性好的高效液相-紫外检测法(HPLC-UV)。色谱条件:分析柱:Agilent Zorbax RX-C8(4.6mm×250mm,5μm);柱温:室温;流动相:乙腈-NaAc缓冲液(0.1mol·L-1,HAc调节pH5.0)=45∶55,流速0.9mL·min-1,检测波长291nm。结果阿苯达唑及其代谢物阿苯达唑亚砜、阿苯达唑砜的血药浓度线性范围分别为10～600,10～1000,10～300μg·L-1;方法回收率分别为92.23%～103.81%,99.59%～100.21%,96.89%～106.84%。批内精密度分别为2.03%～3.12%,2.05%～3.80%,2.79%～4.14%;批间精密度分别为3.20%～4.33%,2.05%～3.48%,2.87%～3.87%。结论本法灵敏、准确,可用于阿苯达唑的人体药动学研究。
OBJECTIVE To establish a method for the determination of albendazole and its metabolites albendazole sulfoxide and albendazole sulfone in plasma by RP-HPLC. Methods Based on the improved literature, HPLC-C2 was used to treat plasma. The HPLC-UV method was developed for the determination of plasma samples with high precision and sensitivity, reproducibility and specificity. Column: Agilent Zorbax RX-C8 (4.6 mm × 250 mm, 5 μm); Column temperature: room temperature; Mobile phase: Acetonitrile-NaCl buffer (0.1 mol·L -1, HAc pH 5.0) = 45: 55, flow rate 0.9mL · min-1, detection wavelength 291nm. Results The linear range of albendazole and its metabolites albendazole sulfoxide and albendazole sulfone were 10 ~ 600, 10 ~ 1000 and 10 ~ 300μg · L-1, respectively. The recoveries were 92.23% ~ 103.81%, 99.59% ~ 100.21%, 96.89% ~ 106.84%. The intra-assay precision was 2.03% -3.12%, 2.05% -3.80% and 2.79% -4.14% respectively. The inter-batch precision was 3.20% -4.33%, 2.05% -3.48% and 2.87% -3.87% respectively. Conclusion This method is sensitive and accurate and can be used in human pharmacokinetics of albendazole.