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目的:制备重楼总皂苷长循环脂质体,并对其进行表征。方法:以粒径、Pd I、Zeta电位及包封率为评价指标,考察制备重楼总皂苷长循环脂质体的各个因素。并采用Malvern粒度仪测定脂质体的粒径分布、Pd I及Zeta电位,透射电镜考察其形态,并考察长脂质体的稳定性。结果:重楼总皂苷长循环脂质体的平均粒径为(109.4±32.7)nm,Pd I为(0.171±0.036),Zeta电位为(-36.7±4.5)m V,包封率为(93.5±3.2)%;透射电镜显示脂质体粒径均一,成球状分布;长期稳定性研究表明,长循环脂质体在4℃条件下放置3个月稳定。结论:重楼总皂苷长循环脂质体制备工艺简单易行,可以得到包封率高、稳定性好的脂质体制剂。
OBJECTIVE: To prepare long-circulating liposomes of total saponins from Rebstock and characterize them. Methods: With the particle size, Pd I, Zeta potential and entrapment efficiency as the evaluation index, the various factors of preparing long circulating liposomes of total reed rhizome were investigated. The particle size distribution, Pd I and Zeta potential of liposomes were determined by Malvern particle size analyzer. The morphology of liposomes was investigated by transmission electron microscopy and the stability of long liposomes was investigated. Results: The average size of the total liposomes of total saponins in Rebridol was (109.4 ± 32.7) nm, the average value of Pd I was (0.171 ± 0.036) and the zeta potential was (-36.7 ± 4.5) m V, ± 3.2%). Transmission electron microscopy showed that the liposomes were uniform in size and distributed in a spherical shape. Long-term stability studies showed that long-circulating liposomes were stable at 4 ℃ for 3 months. CONCLUSION: The preparation method of total saponin long circulating liposomes is simple and easy, and the liposome preparation with high encapsulation efficiency and good stability can be obtained.