论文部分内容阅读
目的:探讨P16抑癌基因与卵巢浆液性癌发生、发展及其生物学特征的关系.方法:应用非放射性原位杂交技术对47例卵巢上皮性浆液性肿瘤组织(良性10例、交界性4例、癌33例)和8例正常卵巢组织中P16MR－NA进行检测.结果:P16MRNA表达主要位于胞浆内呈红色颗粒状,阳性表达细胞多呈灶状分布.P16MRNA在卵巢浆液性癌中检出率(48.5％),低于良性浆液性瘤(90％)及正常卵巢组织(100％)(P均＜0.05).结论:P16MR－NA低表达与卵巢浆液性癌的恶性程度高、肿瘤分化差、癌瘤播散及淋巴结转移有关(P＜0.05或P＜0.01).P16作为一种新型抑癌基因,其MRNA低表达可能与卵巢浆液性癌发生、发展密切相关.“,”Objective:Our purpose was to probe into the relation between tumor suppressor gene p16 and generation and development of ovarian epithelial serous tumors. Methods: By use of in situ hybridization, the tumor suppressor gene p16 mRNA was detected in 47 samples of ovarian serous tumors (10 samples of benign,4 samples of borderline, 33 samples of carcinoma) and 8 samples of normal ovarian tissues. Results: p16 mRNA positive red granules were mainly intracytoplasmic findings. The positive cells were chiefly distributed in focuces. The positive expression rate of p16 mRNA in ovarian epithelial serous carcinoma were 48.5% which was lower than that in benign (90%) and that in normal ovarian tissues (100%, P<0.05). Conclusion:Low expression of p16 mRNA was significantly associated with a higher degree of ovarian serous carcinoma, poorly differentiated, invasiveness and implantation of carcinoma and lymph nodes metastasis (P<0.05 or P<0.01). The results suggested that, low expression of p16 (a novel tumor suppressor gene) were well related with the generation and development of ovarian serous carcinoma.