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利用25对核心引物对中棉所48及其亲本进行多态性检测,共有16对引物在2个亲本间具有多态性,其中,有14对引物在两亲本间扩增出大小不同的带,且这些标记位点在F1中均表现为杂合带,为共显性标记;另外2个引物的F1带型与母本或父本一致,为显性标记。以这25对引物在不同材料间扩增得到的01(二进制)数据转换成十进制数据,构建了中棉所48的数字指纹图谱,为杂交种的真伪鉴定和纯度检测提供了方法。为了构建准确、稳定和实用的棉花DNA指纹图谱,指出了需要规范分子标记类型和数量,DNA、Taq酶等试剂数量和质量,统一PCR反应,电泳和染色条件,统一提供标准品种和标准DNA,标准化电泳条带的识别、数据处理方式和编码方式。
Twenty-five pairs of primers were used to detect polymorphism in Zhongmian48 and its parents. A total of 16 pairs of primers were polymorphic between the two parents. Among them, 14 pairs of primers amplified different size bands , And these marker sites showed heterozygous bands in F1, which were co-dominant markers. The other two primers were consistent with the female or male parent and dominant markers. The 01 (binary) data obtained by amplification of the 25 pairs of primers from different materials were converted into decimal data to construct the digital fingerprinting map of Zhongmiansui 48, which provided a method for authenticity and purity detection of hybrids. In order to establish an accurate, stable and practical cotton DNA fingerprinting profile, it is pointed out that it is necessary to standardize the types and quantities of molecular markers, the quantity and quality of reagents such as DNA and Taq enzyme, uniform PCR reaction, electrophoresis and dyeing conditions, provide standard varieties and standard DNAs uniformly, Standard electrophoresis band identification, data processing methods and coding methods.