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MYB转录因子超家族很多基因参与调控生物钟。本文利用RACE技术克隆到一个中间锦鸡儿MYB家族基因CiMYB177,其c DNA全长2 028 bp,可以编码433个氨基酸。序列分析发现CiMYB177属于CCA1(CIRCADIAN AND CLOCK ASSOCIATED1)亚家族,与大豆的GmMYB177一致性最高(70.3%)。用染色体步移技术克隆到该基因起始密码子上游1 127 bp的启动子序列。用实时荧光定量PCR技术对CiMYB177的转录水平进行分析发现其表达呈节律性的昼夜变化,并且受干旱、NaCl和冷的诱导。亚细胞定位观察发现CiMYB177定位到细胞核。
Many MYB transcription factor superfamily genes are involved in the regulation of the circadian clock. In this paper, a MYC family gene CiMYB177 of Caragana intermedia was cloned by RACE technique. The full-length c DNA of it is 0282 bp, encoding 433 amino acids. Sequence analysis revealed that CiMYB177 belongs to the CCA1 (CIRCADIAN AND CLOCK ASSOCIATED1) subfamily, which has the highest identity (70.3%) to that of soybean (GmMYB177). A 1 127 bp promoter sequence upstream of the gene start codon was cloned by chromosome walking technique. Real-time quantitative PCR analysis of CiMYB177 transcriptional level was found to be circadian rhythmic diurnal changes, and was induced by drought, NaCl and cold. Subcellular localization observed CiMYB177 localization to the nucleus.