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作者通过多位点酶电泳(MLEE)对5种恶性疟原虫(法属圭亚那有Bobo1和Bobo2两种,苏丹和刚果各一种,另一种由5大洲15个不同地区组成的“全球性”样本)作种群基因分析。刚果种群加用随机扩增多态性DNA(RAPD)、限制性长度多态性(RFLP)和4个抗原基因分析。用同工酶研究了编码酶的基因位点,其中刚果、苏丹及“全球性”样本作了12种酶的位点分析,Bobo1、Bobo2分别作了9种和7种酶的位点分析。DNA从
The authors tested the genetic diversity of five species of Plasmodium falciparum by multilocus enzyme electrophoresis (MLEE) (French Guyana has two types of Bobo1 and Bobo2, one each from the Sudan and the Congo, and another one from the 15 different regions of the five continents) Sample) for population gene analysis. Congo populations were analyzed by random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and 4 antigenic genes. The isozyme was used to study the loci encoding enzymes. Conidia, Sudan and “global” samples were analyzed for 12 loci. Bobo1 and Bobo2 were used for loci analysis of 9 and 7 enzymes, respectively. DNA from