论文部分内容阅读
目的 建立稳定表达BMPⅡ型突变受体的Tca8113细胞,以便进一步从信号转导水平探讨、认识BMPs对口腔上皮恶变的作用机理;方法 用FuGENE6 Transfection Reagent Kit真核转染试剂盒将携带BMPsⅡ型突变受体cDNA的真核表达载体转染Tca8113细胞;结果 得到抗G-418的阳性细胞克隆转染细胞,neo基因原位杂交阳性;结论 建立了稳定表达骨形成蛋白(BMPs)Ⅱ型突变受体的Tca8113细胞株。
OBJECTIVE: To establish Tca8113 cells stably expressing BMPⅡtype mutant receptor in order to further explore the mechanism of BMPs on oral malignant transformation. Methods FuGENE6 Transfection Reagent Kit eukaryotic transfection kit, The recombinant plasmid was transfected into Tca8113 cells. The positive cells were transfected with anti-G-418 positive cells and the neo gene was positive by in situ hybridization. Conclusion The stable expression of BMPs Ⅱ mutants was established Tca8113 cell line.