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目的:进行人工诱导衰老糖尿病大鼠模型的初筛。方法:选用Wistar雌性大鼠,采用腹腔注射D-半乳糖后,给予不同周期高脂高糖饲料喂养,再配以不同剂量的链脲佐菌素(STZ)素腹腔注射,制备模型大鼠。检测空腹血糖(FBG)、超氧化物歧化酶(SOD)、丙二醛(MDA),并取胰腺组织行HE染色常规病理切片,以初筛实验性衰老糖尿病大鼠模型。结果:①各组成模率之间差异无显著性;②衰老糖尿病各组大鼠FBG升高,SOD下降,MDA升高;③胰腺病理切片显示:衰老糖尿病各组胰岛细胞团为正常对照组的22.93%-44.51%(P<0.01),且胰岛边界不清,岛内细胞排列不规则,细胞空泡化,细胞数目明显减少。结论:①不同造模法均可达到较高成模率。②采用腹腔注射D-半乳糖42d+高脂高糖6周+腹腔注射STZ(25mg/体质量.体表面积,2次)法所构建的实验性衰老糖尿病模型较为理想。
OBJECTIVE: To screen a rat model of artificially induced diabetes mellitus. Methods: Wistar female rats were selected and intraperitoneally injected with D-galactose. The rats were fed with high-fat and high-sugar diet of different periods and then injected with streptozotocin (STZ) intraperitoneally to prepare model rats. The fasting blood glucose (FBG), superoxide dismutase (SOD) and malondialdehyde (MDA) were detected and the normal pathological sections of the pancreas were obtained by HE staining to screen the experimental aged diabetic rat model. Results: ①No significant difference was found in the compositional rates of each group. ② FBG increased, SOD decreased and MDA increased in all groups of aging diabetic rats. ③ The pathological sections of pancreatic islets showed that the islet cells in the aging diabetic group were normal control group 22.93% -44.51% (P <0.01), and the islet border was unclear. The cells in the island were irregularly arranged and the cells were vacuolized. The number of cells was significantly reduced. Conclusion: ① different modeling methods can achieve a higher modulus. ② The model of experimental aging diabetes was constructed by intraperitoneal injection of D-galactose 42d + high-fat and high-glucose 6 weeks + intraperitoneal injection of STZ (25mg / body weight, body surface area, twice) method.