免疫组织化学是利用免疫反应以定位组织中某类抗原成份分布的一门新技术。近年来,免疫组织化学虽然已经广泛用于一般病理学的研究,但在耳科学领域内尚处于萌芽阶段。尽管1976年即有人报告中耳粘膜溶菌酶的免疫组化定位,但迄今在科研实践中应用此项先进技术者不多。加以内耳组织制片上的困难,内耳免疫组化研究的发展更晚,80年代才开始报告这一方面的资料。作者从1981年起即在美国马萨诸塞总医院及眼耳鼻喉医院研究此一技术,1985年在第十三届世界 Immunohistochemistry is a new technique that utilizes the immune response to locate the distribution of a group of antigens in tissues. In recent years, although immunohistochemistry has been widely used in general pathology, but in the field of otology is still in its infancy. Although the immunohistochemical localization of mucosal lysozyme in the middle ear was reported in 1976, little has been done so far in the application of this advanced technology in scientific research. The difficulties in the preparation of the inner ear tissue have led to the development of immunohistochemical studies of the inner ear later in the 1980s. The authors studied this technique at the Massachusetts General Hospital and Eye, Ear, Nose and Throat Hospital in the United States since 1981, in the thirteenth world