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Objective To investigate the association between plasminogen activator inhibitor(PAI)-2 expression and invasive potential in hepatocellular carcinoma(HCC) cells.Methods The HCC cell lines with high,low,and non-metastatic potentials,namely MHCC97-H,MHCC97-L,and SMMC-7721 respectively,were cultured in vitro.Matrigel invasion assay and Western blot of PAI-2 protein expression were conducted.Results The number of invaded cells in MHCC97-L was significantly higher than that in SMMC-7721(P=0.005),whereas that in MHCC97-H was higher than in MHCC97-L(P=0.017) and SMMC-7721(P=0.001).Contrarily,PAI-2 protein expression was gradually reducing from SMMC-7721,MHCC97-L,to MHCC97-H(MHCC97-H vs.MHCC97-L,P<0.001;MHCC97-H vs.SMMC-7721,P=0.001;MHCC97-L vs.SMMC-7721,P=0.001).The Pearson’s correlation analysis revealed a significant negative association between invaded cell number and PAI-2 expression(r= 0.892,P=0.001).Conclusion PAI-2 expression may be negatively associated with the invasive potential of HCC.
Objective To investigate the association between plasminogen activator inhibitor (PAI) -2 expression and invasive potential in hepatocellular carcinoma (HCC) cells. Methods The HCC cell lines with high, low, and non-metastatic potentials, for MHCC97-H, MHCC97- , and SMMC-7721 respectively, were cultured in vitro. Matrigel invasion assay and Western blot of PAI-2 protein expression were performed. Results The number of invaded cells in MHCC97-L was significantly higher than that in SMMC-7721 ), that that MHCC97-H was higher than MHCC97-L (P = 0.017) and SMMC-7721 (P = 0.001) .Contrarily, PAI- MHCC97-H vs. MHCC97-L, P <0.001; MHCC97-H vs. SMMC-7721, P = 0.001; MHCC97-L vs. SMMC-7721, P = 0.001) .The Pearson’s correlation analysis revealed a Significant negative association between invaded cell number and PAI-2 expression (r = 0.892, P = 0.001) .Conclusion PAI-2 expression may be negatively associated with the invasive potential of HCC.