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应用[~3H]TdR掺入离体培养大鼠肝细胞DNA的方法,测定由本室提取的pHSS的生物活性。结果表明,pHSS可显著促进原代培养大鼠肝细胞的DNA合成,其促进率约为对照组的10倍左右。培养液中血清浓度对pHSS的生物活性表达有显著影响,不同浓度血清可以使pHSS表现出不同的量效关系,这些结果在Buffello大鼠肝细胞系的实验中得到进一步证实。在低剂量pHSS的刺激下,不同年龄大鼠肝细胞的[~3H]TdR掺入率无显著差异。但高剂量时,pHSS对幼鼠作用不明显。
The method of [~ 3H] TdR incorporation into ex vivo rat hepatocyte DNA was used to determine the biological activity of pHSS extracted from this chamber. The results showed that pHSS significantly promoted the DNA synthesis of primary cultured rat hepatocytes, and its promotion rate was about 10 times that of the control group. The serum concentration of culture medium had a significant effect on the biological activity of pHSS. Different concentrations of serum could make pHSS show different dose-effect relationship. These results were further confirmed in the experiment of Buffello rat hepatocyte line. Under the stimulation of low dose of pHSS, [~ 3H] TdR incorporation rate of hepatocytes in different age rats showed no significant difference. However, the effect of pHSS on young rats was not obvious at high dose.