论文部分内容阅读
目的:探讨癫痫对大鼠海马线粒体细胞色素氧化酶(COX)的mtDNA和nDNA编码亚基Ⅲ、Ⅳ表达的影响。方法:将成年雄性Wistar大鼠40只随机分为生理盐水对照组,癫痫持续状态(SE)后急性期(3 h)、静止期(7 d)、慢性期(45 d)组和注射匹鲁卡品(PILO)但未出现SE组,每组8只。荧光实时定量PCR和West-ern blot分别检测海马线粒体COXⅢ、ⅣmRNA和蛋白的表达。结果:COXⅢmRNA和蛋白在SE后3 h表达明显升高(P<0.001,P<0.01),7 d时降到对照水平,45 d显著降低(P<0.001,P<0.01);COXⅣmRNA和蛋白在3 h时表达较对照组升高,但差异无统计学意义(P>0.05),7 d和45 d时下降至对照水平。注射PILO但无SE组与对照组结果类似。结论:颞叶癫痫海马cox功能紊乱与痫性发作相关,线粒体编码的基因更易受痫性发作的影响。
Objective: To investigate the effects of epilepsy on mitochondrial cytochrome oxidase (COX) mtDNA and nDNA coding subunits Ⅲ, Ⅳ in rat hippocampus. Methods: Forty adult male Wistar rats were randomly divided into three groups: normal saline control group, acute phase (3 h), quiescent phase (7 d), chronic phase (45 d) and epileptic seizure group PILO but no SE group, 8 in each group. Fluorescence real-time quantitative PCR and West-ern blot were used to detect the expression of COX Ⅲ, Ⅳ mRNA and protein in hippocampal mitochondria. Results: The mRNA and protein expression of COX Ⅲ increased significantly at 3 h after SE (P <0.001, P <0.01), decreased to the control level on the 7 d, decreased significantly at 45 d (P <0.001, P <0.01) Compared with the control group, the expression increased at 3 h, but the difference was not statistically significant (P> 0.05). At 7 d and 45 d, the expression decreased to the control level. PILO injection but no SE group with the control group similar results. CONCLUSIONS: Cox dysfunction in the temporal lobe epilepsy is associated with seizures and mitochondrial-encoded genes are more susceptible to seizures.