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ＳｐｒａｇｕｅＤａｗｌｅｙ大鼠２８只，随机分为４组：①对照组：ｎ＝１０，大鼠腹腔注射（ｉｐ）生理盐水（ＮＳ）２ｍｌ·ｋｇ－１；②利血平组：ｎ＝６，大鼠ｉｐ利血平２．０ｍｇ·ｋｇ－１；③苯海索组：ｎ＝６，大鼠ｉｐ苯海索３．０ｍｇ·ｋｇ－１，每隔４ｈ给药１次，共５次；④苯海索加利血平组：ｎ＝６，大鼠ｉｐ苯海索３．０ｍｇ·ｋｇ－１，每隔４ｈ给药１次，共５次，最后一次给药时同时ｉｐ利血平２．０ｍｇ·ｋｇ－１。各组动物均在最后一次给药后１ｈ断头取脑，分出皮层、海马、间脑和脑干。荧光法测定各脑区单胺递质含量。单胺递质包括去甲肾上腺素（ＮＥ）、多巴胺（ＤＡ）、５羟色胺（５ＨＴ）和５羟吲哚乙酸（５ＨＩＡＡ）。结果：①利血平使大鼠皮层、脑干、间脑和海马ＮＥ、ＤＡ、５ＨＴ显著下降，５ＨＩＡＡ显著升高；②苯海索使大鼠皮层、脑干、间脑和海马ＮＥ、ＤＡ、５ＨＴ和５ＨＩＡＡ显著升高；③苯海索和利血平联合用药后，大鼠间脑ＤＡ、海马ＮＥ显著升高，皮层、脑干、间脑和海马５ＨＩＡＡ显著升高。结果提示：利血平可以耗竭脑内单胺递质，苯海索可以提高脑内单胺递质的浓度，苯海索可以拮抗利血平耗竭脑内单胺递质的作用。
28 Sprague-Dawley rats were randomly divided into 4 groups: ① control group: n = 10, rats were intraperitoneally injected with NS 2ml · kg-1; ② reserpine group: n = 6, Rats were given ip reserpine 2.0mg · kg-1; ③ triamcinolone group: n = 6, rat ip trihydroxybenzoate 3.0mg · kg-1, once every 4h, 5 times; (4) The rats in benhexisalgylate level were n = 6, rats were given tributylprednisolone 3.0 mg · kg-1 once every 4 hours for 5 times. At the same time, 2.0 mg · kg -1. Animals in each group were decapitated 1h after the last administration, and the cortex, hippocampus, diencephalon and brainstem were separated. Fluorescence determination of monoamine neurotransmitters in brain regions. Monoamine neurotransmitters include NE, DA, 5-HT and 5-HIAA. Results: ① Reserpine remarkably decreased NE, DA and 5HT in rat cortex, brainstem, diencephalon and hippocampus, while 5HIAA increased significantly. ② Phenoxyethanol significantly decreased the levels of cortisol, brainstem, Hippocampal NE, DA, 5 HT and 5 HIAA increased significantly; â’¡ benzodiazepine and reserpine combined treatment of rat brain DA, hippocampus NE was significantly increased cortex, brainstem, diencephalon and hippocampus 5 HIAA increased significantly. The results suggest that reserpine can deplete monoamine neurotransmitters in the brain, and that trihexyphenidyl may increase monoamine neurotransmitters in the brain, and that trihexyphenidyl may antagonize reserpine depletion of monoamine neurotransmitters in the brain.