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目的 :研究渗透性应激对神经元凋亡的诱导。方法 :体外原代培养鸡胚端脑神经元 ,以 1mol·L-1山梨醇处理神经元 1h ,换正常营养液 ,1h后行倒置显微镜、透射电镜形态学观察 ,MTT(噻唑蓝 )细胞活性分析以及DNA琼脂糖凝胶电泳。结果 :神经元活性明显下降 ,DNA电泳及形态学观察显示神经元以凋亡的形式死亡。结论 :渗透性应激可以诱导体外原代培养鸡胚端脑神经元凋亡
Objective: To study the induction of neuronal apoptosis by osmotic stress. Methods: Primary cultured chick embryonic neurons were cultured in vitro with 1 mol·L-1 sorbitol for 1 h and switched to normal nutrient solution. After 1 h, inverted microscope was used to observe the morphological changes of MTT (thiazolyl blue) cells Analysis and DNA agarose gel electrophoresis. Results: Neuronal activity decreased significantly. DNA electrophoresis and morphological observation showed that neurons died in the form of apoptosis. CONCLUSION: Osmotic stress can induce apoptosis of primary cultured embryonic neurons in chicken embryos