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目的：观察钙拮抗剂维拉帕米、硝苯地平、恬而心，在不同浓度时对系膜细胞增殖及肥大的作用。方法：体外对人体系膜细胞进行二维培养，选第４～６代系膜细胞置９６孔培养板进行研究。用促有丝分裂剂ＰＤＧＦ（５μｇ／Ｌ）、凝血酶（５ＮＩＨｋＵ／Ｌ）促其增殖，加或不加三种不同类型钙拮抗剂：维拉帕米、硝苯地平、恬而心，观察对以上刺激的抑制作用，加入同位素３ＨＴｄＲ（１μＣｉ／孔）、３ＨＵｒ（１μＣｉ／孔）、３ＨＬｅｕ（１μＣｉ／孔），液闪计数仪测定ｍｉｎ１值。结果：经ＰＤＧＦ、凝血酶刺激后３ＨＴｄＲ掺入系膜细胞的ｍｉｎ１值分别为６０７０±５１０及４２５０±５２０。当加入１０５ｍｏｌ／Ｌ维拉帕米、硝苯地平、恬而心时，其ｍｉｎ１值分别为３３３０±３７０和２７８０±１１０，４５８０±４２０和３０３０±３６０，４０００±４６０和３０９０±２８０（Ρ均＜００５）。而经ＰＤＧＦ、凝血酶刺激后，３ＨＵｒ、３ＨＬｅｕ掺入系膜细胞的ｍｉｎ１值在加入１０５ｍｏｌ／Ｌ维拉帕米、硝苯地平及恬而心前后差异不大（Ρ均＜００５）。结论：较高浓度的钙拮抗剂均能抑制由ＰＤＧＦ、凝血酶诱导的系膜细胞增殖，而对其肥大没有影响。其理论机理?
Objective: To observe the effects of verapamil and nifedipine, a calcium antagonist, on the proliferation and hypertrophy of mesangial cells at different concentrations. Methods: Two-dimensional culture of human mesangial cells was performed in vitro. The mesangial cells from the 4th to 6th passages were placed in 96-well plates. Proliferation was induced with the mitogenic agent PDGF (5 μg / L) and thrombin (5 NIHkU / L), with or without three different types of calcium antagonists: verapamil, nifedipine, Stimulate the inhibition, adding isotope 3H TdR (1μCi / hole), 3H Ur (1μCi / hole), 3H Leu (1μCi / hole), measured by liquid scintillation count 1 value. Results: After PDGF, thrombin 3H thymidine incorporation of cell membrane 1 values were 6070 ± 510 and 4250 ± 520. When added 10 5mol / L verapamil, nifedipine, Tian heart, the min 1 values were 3330 ± 370 and 2780 ± 110,4580 ± 420 and 3030 ± 360,4000 ± 460 and 3090 ± 280 (all P <005). The PDGF, thrombin stimulation, 3H Ur, 3H Leu incorporation of mesangial cells min 1 value in 10 5mol / L verapamil, nifedipine and before and after Tian Tian little difference ( P <005). CONCLUSION: Higher concentrations of calcium antagonist can inhibit the proliferation of mesangial cells induced by PDGF and thrombin, but have no effect on the hypertrophy. Its theoretical mechanism?