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用随机扩增多态DNA(RAPD)方法对红麻种质资源的遗传变异和亲缘关系进行分析。6个引物对14份日本使用的红麻品种扩增出总共30条RAPD多态性条带。根据RAPD图谱模式可将红麻品种区分开来,区分范围从6份(用OPA-20引物扩增)到12份(用OPA-11引物扩增)的品种。用这30条多态性条带构建出的聚类分析树状图,将这14份红麻品种聚成源于印度、中国、越南的三大类。在随后进行的用四个引物对19份从美国引入的红麻种质资源的RAPD分析中,获得以35条多态性条带为基础的RAPD图谱并构建了树状图。结果认为这19份材料的大部分源于EISalvador种系。起源相近或亲本来源相同的红麻品种资源随着时间、环境和人为等因素的影响农艺性状不断变异,但在DNA分子水平上的变异相对较小。根据红麻的农艺性状等很难判定品种的来源,而RAPD方法可区分鉴定品种以及明确品种资源间的亲缘关系。
Random amplified polymorphic DNA (RAPD) method was used to analyze the genetic variation and genetic relationship of kenaf germplasm resources. Six primers amplified a total of 30 RAPD polymorphic bands in 14 kenaf varieties used in Japan. The kenaf varieties can be distinguished according to the RAPD pattern pattern, ranging from 6 parts (amplified with OPA-20 primers) to 12 (amplified with OPA-11 primers). Using the 30 polymorphic bands to construct a cluster analysis tree map, these 14 kenaf varieties were clustered into three major categories from India, China and Vietnam. In the subsequent RAPD analysis of nineteen kennel germplasm resources introduced from the United States with four primer pairs, a RAPD pattern based on 35 polymorphic bands was obtained and a dendrogram was constructed. As a result, most of these 19 materials were derived from the EISalvador line. The kenaf varieties with similar or similar origin originated from the same source had different agronomic traits with time, environment and man-made factors, but the variation in DNA molecular level was relatively small. According to the kenaf agronomic traits such as difficult to determine the source of varieties, and RAPD method can distinguish between identified varieties and clear genetic relationship between varieties of resources.