目的探讨ｎｍ２３Ｈ１基因表达与胃癌生物学行为及淋巴结转移规律的关系。方法应用免疫组织化学方法观察了ｎｍ２３Ｈ１基因产物／核苷二磷酸激酶（ｎｍ２３／ＮＤＰＫ）在７５例胃癌组织中的表达。结果胃癌ｎｍ２３／ＤＮＰＫ表达阳性率为５９％，在浸透浆膜（Ｔ３、Ｔ４），淋巴结转移５个以上及Ⅱ站以远者，Ⅲ、Ⅳ期和伴腹膜转移的胃癌中表达明显减低（Ｐ＜００５）。在不同大体类型、分化程度和生长方式的胃癌中，ｎｍ２３Ｈ１表达差异无显著意义（Ｐ＞００５）。ｎｍ２３Ｈ１阳性组和阴性组５年生存率分别为６１％和３５％，二者差异有显著意义（Ｐ＜００５）。结论ｎｍ２３Ｈ１基因表达与胃癌浸润转移和预后有关，对评估淋巴结转移程度可能有重要意义 Objective To investigate the relationship between the expression of nm23H1 gene and the biological behavior and lymph node metastasis of gastric cancer. Methods The expression of nm23H1 gene product/nucleotide diphosphate kinase (nm23/NDPK) in 75 gastric cancer tissues was observed by immunohistochemistry. Results The positive rate of nm23/DNPK expression in gastric cancer was 59%. The expression of nm23/DNPK was significantly decreased in sero-membrane (T3, T4), lymph node metastasis of more than 5 and II stations, and in stage III, IV and peritoneal metastasis. <005). There was no significant difference in the expression of nm23-H1 among gastric cancers of different general types, differentiation degrees and growth patterns (P>005). The 5-year survival rates of the nm23-H1 positive and negative groups were 61% and 35%, respectively, and there was a significant difference between the two groups (P < 0.05). Conclusion The expression of nm23H1 gene is related to the invasion, metastasis and prognosis of gastric cancer, and may be of significance in evaluating the degree of lymph node metastasis.