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目的:获得IL2/HBVpreS融合基因克隆,为表达IL2/HBVpreS融合蛋白奠定基础。方法:用PCR方法从乙肝全序列中扩增HBVpreS基因片段,并克隆入带有IL2基因的ply5质粒中,挑出的阳性克隆再亚克隆到pUC18中进行序列测定。结果:基因全长930bp,编码310个氨基酸,序列内有起始码和终止码。结论:所克隆的基因为IL2与HBVpreS的融合基因。
Objective: To obtain IL 2 / HBVpreS fusion gene clone, to lay the foundation for the expression of IL 2 / HBVpreS fusion protein. Methods: The HBV preS gene fragment was amplified by PCR from the complete HBV genome and cloned into ply5 plasmid with IL2 gene. The positive clones were subcloned into pUC18 for sequencing. Results: The gene was 930 bp in length and encoded a protein of 310 amino acids with a start code and a stop codon in the sequence. Conclusion: The cloned gene is a fusion gene between IL2 and HBVpreS.