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应用荧光双标组织化学技术观察到，在大鼠腰段脊神经节中，GriffoniasimplicifoliaI-B.（I-B4）结合反应物与P物质样免疫反应物均分布在小细胞中，且34％～43％的I-B4结合反应阳性细胞和87％～95％的P物质阳性细胞为I-B4与P物质双标细胞。切断单侧脊神经后根的大鼠，切断侧脊髓后角内I-B4结合反应在术后第3d即明显减弱，至第5d消失。向成年大鼠蛛网膜下腔注射辣椒素后，脊髓后角和Lissauer束内的I-B4结合反应于第3d已明显减弱，于第5d、第7d和第10d减弱更显著，但仍有部分残留。电镜下.脊髓后角I～III层有大量轴突终末被I-B4标记，其中许多标记终末参与构成突触小球。在包埋前免疫金-银-ABC法双标电镜标本上，脊髓后角内I-B4与SP双标轴突终末分别占I-B4阳性轴突终未总数和P物质阳性轴突终末总数的33％～36％和39％～53％。上述结果进一步证实了I-B4与初级传入的小神经元结合的特异性，并表明I-B4结合位点是鉴别脊髓后角内P物质阳性轴突终末的初级传入来源的重要标志。
It was observed by fluorescence double-stained histochemical techniques that Griffoniasimplicifolia I-B. (I-B4) binding substance and substance P-like immunoreactive substance were distributed in small cells in rat lumbar spinal ganglia and 34% -43 % Of I-B4 binding positive cells and 87% -95% of substance P positive cells are double labeled I-B4 and substance P cells. Rats in the dorsal root of the unilateral spinal nerve were cut off, and the I-B4 binding reaction in the dorsal horn of the severed lateral spinal cord decreased significantly on the 3rd day and disappeared on the 5th day. After injection of capsaicin into the subarachnoid space of adult rats, the I-B4-binding reaction in the spinal cord and Lissauer bundle decreased significantly on the 3rd day and weakened more significantly on the 5th, 7th and 10th day, but there was still some Residual. Under the electron microscope, a large number of axon terminals were labeled with I-B4 in the posterior horn of spinal cord, many of which were involved in the formation of synaptic globules. In the pre-embedding immuno gold-silver-ABC double-labeled electron microscopy, the I-B4 and SP double-labeled axon terminals in the dorsal horn of the spinal cord accounted for the total number of I-B4-positive axons and the end of the substance P-positive axon, respectively The total number of the end of 33% to 36% and 39% to 53%. The above results further confirm the specificity of I-B4 binding to primary afferent small neurons and suggest that the I-B4 binding site is an important marker for identifying the primary afferent source of substance P-positive axon termini in the posterior horn of the spinal cord .