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目的:研究新疆紫草水溶性提取物抗HIV-1体外活性。方法:新疆软紫草干燥根,先用正己烷脱脂后,再用丙酮水溶液提取,水溶部分分别用乙酸乙酯和正丁醇除去中强极性成分,余下的水溶液层经过MCI-GEL CHP20P、Sephadex LH-20分离,用甲醇-水洗脱,得4个提取组分(Ⅰ、Ⅱ、Ⅲ和Ⅳ)。将该组分分别与MT-2细胞、HIV-1ⅢB共培养,采用MTT染色法,观察对HIV-1感染细胞的保护作用和对MT-2细胞的毒性作用。结果:组分Ⅰ、Ⅱ、Ⅲ、Ⅳ对HIV-1感染MT-2细胞的50%有效浓度(EC50)分别5.0、10.0、2.8和6.3μg/ml,50%抑制细胞生长浓度(TC50)为640、160、180和400μg/ml,治疗指数(SI)为128、16、64和63。结论:新疆紫草水溶性提取物对HIV-1感染MT-2细胞具有保护作用,同时对正常细胞也有一定的毒性,组分Ⅲ活性最强,Ⅱ毒性最强,值得进一步研究。
Objective: To study the anti-HIV-1 activity of Arnebia euchroma (Royle) rhizome aqueous extract in vitro. Methods: The roots of Xinjiang soft shelled shrubs were degreased with n-hexane and then extracted with acetone. The water-soluble fractions were neutralized by ethyl acetate and n-butanol. The remaining aqueous solutions were separated by MCI-GEL CHP20P and Sephadex LH-20 was separated and eluted with methanol-water to give 4 fractions (I, II, III and IV). The components were co-cultured with MT-2 cells and HIV-1ⅢB. MTT staining was used to observe the protective effect on HIV-1 infected cells and the cytotoxicity on MT-2 cells. Results: The 50% effective concentration (EC50) of components Ⅰ, Ⅱ, Ⅲ and Ⅳ for HIV-1 infected MT-2 cells were 5.0, 10.0, 2.8 and 6.3μg / ml respectively and the 50% inhibitory concentration (TC50) 640, 160, 180 and 400 μg / ml, therapeutic index (SI) 128, 16, 64 and 63. Conclusion: Xinjiang Arnebiae Radix water soluble extract has protective effect on HIV-1 infected MT-2 cells, and also has certain toxicity to normal cells. It has the strongest activity of component Ⅲ and the strongest toxicity of Ⅱ, which deserves further study.