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为建立有效的坛紫菜自由丝状体移植育苗技术,以坛紫菜的2个选育品系“申福1号”和“申福2号”及野生型品系为实验对象,初步研究了自由丝状体的移植量、移植后培养的光密度和温度对贝壳丝状体的生长和壳孢子放散量的影响,结果如下:当自由丝状体移植量为50～500 mg/m2,随着移植量的增加,贝壳丝状体的藻落数显著增加,但移植量如果超过100 mg/m2,贝壳丝状体的壳孢子放散量反而减少。培养光密度在10～50μmol photons/(m2.s)范围内,随着光密度的增加,贝壳丝状体的生长速度明显增快,但壳孢子的放散量却随着光密度的提高而减少。培养温度在15～25℃范围内,随着温度的升高,贝壳丝状体的生长明显加快;在15～20℃范围内,壳孢子放散量随着培养温度的升高而增加,在20～25℃范围内,壳孢子放散量反而随着培养温度的升高而减少。上述结果表明,在坛紫菜自由丝状体移植育苗中,过大的丝状体移植量、过高的培养温度和光密度均会导致贝壳丝状体的壳孢子放散量减少。
In order to establish an effective cultivation technique of algae free larvae transplanting, two experimental lines of seaweed, “Shenfu No.1” and “Shenfu No.2”, as well as wild type strains were studied. The results showed that when the amount of free filamentous grafts was 50 ~ 500 mg / m2, the amount of free filamentous grafts, the optical density and the temperature after transplantation, With the increase of transplantation volume, the number of algae in shellfish filaments increased significantly. However, if the transplantation volume exceeded 100 mg / m2, the conidial spore release decreased. In the range of 10 ~ 50μmol photons / (m2.s), the growth rate of the shell filaments was significantly increased with the increase of optical density, but the release of the hypocotyls decreased with the increase of the optical density . At 15 ~ 25 ℃, with the increase of temperature, the growth of the shell-like filament obviously accelerated. At 15 ~ 20 ℃, the release of conchospores increased with the increase of incubation temperature, In the range of ~ 25 ℃, the conidial spore release decreased with the increase of culture temperature. The above results indicate that excessive filamentous graft, excessive culture temperature and optical density all lead to a decrease in the amount of conidia of conchocelis in seedling culture.