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目的:探讨亚甲蓝/光化学法灭活人血浆中SARS冠状病毒(SARS CoV)的效果。方法:从SARS患者血清中分离出冠状病毒,经VeroE6细胞增殖后将病毒加入含有不同浓度亚甲蓝的人血浆中,再用亚甲蓝/光化学法处理不同时间,取样接种细胞,观察细胞病变作用。结果:在激发光源照度为40000lx条件下,经1μmol/L的亚甲蓝作用30min可灭活血浆中6.5lgTCID50/ml的SARS CoV,经5μmol/L的亚甲蓝作用3min可灭活上述滴度病毒。结论:亚甲蓝/光化学法能够灭活血浆中的SARS CoV。
Objective: To investigate the effect of methylene blue / photochemical inactivation of SARS coronavirus (SARS CoV) in human plasma. Methods: The coronavirus was isolated from the serum of patients with SARS. The virus was added into human plasma containing different concentrations of methylene blue after proliferation by Vero E6 cells. The cells were treated with methylene blue / photochemical method for different time, and the cells were seeded for cytopathic effect effect. Results: Under the illumination of 40 000 lx, SARS CoV of 6.5 lg TCID50 / ml in plasma was inactivated by 1 μmol / L methylene blue for 30 min. The titer was inactivated by 5 μmol / L methylene blue for 3 min virus. Conclusion: Methylene blue / photochemical method can inactivate SARS CoV in plasma.