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目的 :研究羟基聚磷酸钙钠 (HPA)、羟基磷灰石 (HA)、生物玻璃陶瓷 (BGC)和纯钛 (Ti) 4种硬组织替代材料对成骨细胞的细胞内游离钙 ([Ca2 + ]i)浓度、钙调素 (CaM)含量以及细胞膜ATP酶活性的影响 ,探讨这 4种材料的生物相容性。方法 :选用SD乳鼠颅顶骨成骨细胞进行体外培养 ,把HPA、HA、BGC和Ti粉分别制成材料浸提液 ,将培养成活的成骨细胞接种于材料浸提液中培养 ,3d后测定成骨细胞内 [Ca2 + ]i 浓度、CaM含量及细胞膜ATP酶活性。结果 :4种材料对细胞内游离钙浓度无不良影响 ,钛对钙调素含量有明显抑制作用 (P <0 0 5 ) ,HPA、HA、BGC 3种材料对细胞膜Ca2 + ATP酶活性有增强作用 (P <0 0 5 ) ,HPA、HA 2种材料对Na+ 、K+ ATP酶活性有增强作用 (P <0 0 5 )。结论 :[Ca2 + ]i 浓度、CaM含量及细胞膜ATP酶活性能够反映不同硬组织替代材料的生物相容性 ,可以为建立和完善评价硬组织替代材料生物相容性的方法和指标提供依据。
OBJECTIVE: To study the effects of four kinds of hard tissue substitute materials, including HPA, HA, BGC and Ti, on the intracellular free calcium ([Ca2 + +] i) concentration, calmodulin (CaM) content and membrane ATPase activity, to explore the biocompatibility of these four materials. Methods: SD rat osteoblasts were cultured in vitro. The HPA, HA, BGC and Ti powders were made into material extracts respectively. The cultured osteoblasts were inoculated into the material extracts for culture. After 3d The [Ca2 +] i concentration, CaM content and membrane ATPase activity in osteoblasts were determined. RESULTS: The four materials had no adverse effect on intracellular free calcium concentration, but titanium significantly inhibited the calmodulin content (P <0.05). The activities of Ca2 + ATPase in plasma membrane were enhanced by the three materials HPA, HA and BGC (P <0 05), HPA, HA 2 kinds of materials on Na +, K + ATPase activity increased (P <0 05). CONCLUSION: The concentration of [Ca2 +] i, the content of CaM and the activity of membrane ATPase can reflect the biocompatibility of alternative materials in different hard tissues, which may provide the basis for establishing and perfecting the methods and indexes for evaluating the biocompatibility of hard tissue substitute materials.