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目的:研究膳食锌缺乏对生长期大鼠血清各种元素的影响。方法:刚断奶的SD大鼠,按体重相近配伍,随机分为3组,每组8只:缺锌组(ZD),配饲组(PF),对照组(ZA)。缺锌饲料、正常饲料含锌量分别为0.4mg/kg3、0 mg/kg,实验期15 d。用等离子电感耦合质谱仪(ICP—MS)测定大鼠血清元素锌、铁、铜、硒、锰、钴、铬、钙、镁、镉的含量。结果:缺锌组大鼠:血清铁(573.86±201.71μg/L)明显低于配饲组(1372.37±118.69μg/L)和正常组(1463.55±331.66μg/L)(P<0.05);血清锰(12.28±5.33μg/L)明显高于配饲组(4.24±0.73μg/L)和正常组(3.10±1.10μg/L)(P<0.05);血清铜(534.71±113.77μg/L)、硒(262.55±38.57μg/L)、镁(25.26±9.29μg/dl)低于正常组(铜725.91±158.27μg/L,硒465.90±132.18μg/L,镁39.84±14.10μg/dl)(P<0.05),与配饲组无明显差异(铜566.86±134.96μg/L,硒336.71±112.30μg/L,镁29.59±6.99μg/dl)(P>0.05);相比于配饲组与正常组,血清钙有下降趋势,但差异无显著性(P>0.05),血清钴、铬、镉有升高趋势,但差异无显著性(P>0.05)。结论:膳食锌缺乏影响生长期大鼠体内的微量元素水平。
Objective: To study the effects of dietary zinc deficiency on various serum elements in growing rats. Methods: The newly weaned SD rats were randomly divided into three groups according to body weight: 8 for each group: ZD group, PF group and ZA group. Zinc deficiency feed, zinc content of normal feed were 0.4mg / kg3,0 mg / kg, the experimental period of 15 d. Plasma concentrations of zinc, iron, copper, selenium, manganese, cobalt, chromium, calcium, magnesium and cadmium were determined by ICP-MS. Results: Serum iron (573.86 ± 201.71μg / L) was significantly lower in the zinc-deficient group than that in the fed group (1372.37 ± 118.69μg / L) and the normal group (1463.55 ± 331.66μg / L) Manganese (12.28 ± 5.33μg / L) was significantly higher than that of the fed group (4.24 ± 0.73μg / L) and the normal group (3.10 ± 1.10μg / L) , Selenium (262.55 ± 38.57μg / L) and magnesium (25.26 ± 9.29μg / dl) were lower than the normal group (725.91 ± 158.27μg / L, 465.90 ± 132.18μg / L, 39.84 ± 14.10μg / (P <0.05). There was no significant difference between the two groups (P <0.05) In the normal group, the level of serum calcium decreased, but the difference was not significant (P> 0.05). The levels of serum cobalt, chromium and cadmium had no significant difference (P> 0.05). Conclusion: Dietary zinc deficiency affects the levels of trace elements in the growing rats.