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目的:探讨慢性丙型肝炎病毒感染患者HCV RNA增殖状态与T细胞亚群功能及血浆中IL-2和SIL-2R活性的关系。方法:以间接免疫荧光法、ELISA法和RT-PCR分别检测75例慢性HCV感染患者(其中HCV RNA阳性47例,HCV RNA阴性28例)外周血T细胞亚群、IL-2、sIL-2R的水平和HCV RNA。结果:慢性HCV感染患者周围血CD_3~+、CD_4~+淋巴细胞亚群,CD_4~+/CD_3~+比值及IL-2水平均显著低于正常对照组(P<0.01),而sIL-2R明显升高(P<0.01);血清HCV RNA阳性患者T细胞亚群,CD_4~+/CD_3~+比值及IL-2水平显著低于HCV RNA阴性患者(P<0.01)。结论:慢性HCV感染患者存在机体细胞免疫功能低下,HCV RNA阳性患者较阴性患者更甚,提示细胞免疫功能受抑可能是HCV持续增殖的原因。
Objective: To investigate the relationship between the proliferation status of HCV RNA and the function of T lymphocyte subsets and the activity of IL-2 and SIL-2R in plasma in patients with chronic hepatitis C virus infection. Methods: T lymphocyte subsets, IL-2 and sIL-2R in peripheral blood of 75 patients with chronic HCV infection (including 47 HCV RNA positive and 28 HCV RNA negative) were detected by indirect immunofluorescence, ELISA and RT- Level and HCV RNA. Results: The levels of CD_3 ~ +, CD_4 ~ + lymphocyte subsets, CD_4 ~ + / CD_3 ~ + and IL-2 in peripheral blood of patients with chronic HCV infection were significantly lower than those in the normal control (P <0.01) (P <0.01). The level of CD_4 ~ + / CD_3 ~ + and IL-2 in patients with positive HCV RNA were significantly lower than those with negative HCV RNA (P <0.01). CONCLUSIONS: In patients with chronic HCV infection, cellular immunity is poor and HCV RNA positive patients are even worse than negative ones, suggesting that inhibition of cellular immune function may be responsible for the continued proliferation of HCV.