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用圆柱形细胞培养瓶,对草鱼吻端组织ZC—7901细胞适应旋转培养条件进行了研究,测定了细胞接种浓度、旋转速度及细胞生长速度等有关参数。试验表明ZC—7901细胞能适应于旋转培养,适应后的细胞对草鱼出血病病毒仍具有敏感性。
The culture conditions of ZC-7901 cells in grass carp were studied by using cylindrical culture flasks. The parameters such as cell seeding concentration, rotation speed and cell growth rate were determined. Experiments show that ZC-7901 cells can adapt to the rotation culture, the adapted cells are still sensitive to grass carp hemorrhagic disease virus.