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目的观察DC-Chol脂质体DNA复合物(DLDC)体外抗乙型肝炎病毒(HBV)的作用。方法制备DLDC,HepG2.2.15细胞经不同浓度的DLDC作用后,检测其对细胞的毒性作用;提取细胞质DNA,用化学发光Southern blot法检测DLDC对细胞内HBV DNA复制的抑制作用。结果DLDC可抑制HepG2.2.15细胞增殖,其TC0为78.13 ng/ml,TC50为421.86 ng/ml;DLDC可抑制HepG2.2.15细胞HBV DNA复制,其IC50为45 ng/ml,SI为9.37。结论DLDC对胞内HBV DNA的复制有明显的抑制作用。
Objective To observe the effect of DC-Chol liposome DNA complex (DLDC) against hepatitis B virus (HBV) in vitro. Methods DLDC was prepared. HepG2.2.15 cells were treated with DLDC at different concentrations to detect the cytotoxicity of DLDC. Cytoplasmic DNA was extracted and the inhibitory effect of DLDC on HBV DNA replication was detected by chemiluminescence Southern blot. Results DLDC inhibited the proliferation of HepG2.2.15 cells with a TC0 of 78.13 ng / ml and a TC50 of 421.86 ng / ml. DLDC inhibited HBV DNA replication in HepG2.2.15 cells with IC50 of 45 ng / ml and SI of 9.37. Conclusion DLDC has obvious inhibitory effect on intracellular HBV DNA replication.