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以青藏公路沿线土壤为研究对象,研究了土壤可培养微生物数量的变化特征及影响因子.结果表明:青藏公路沿线土壤可培养微生物数量为0.77×106～2.44×107 CFU·g-1dw;沿青藏公路从南(申格里贡山)到北(西大滩),土壤可培养细菌与真菌数量表现为先迅速减少,然后渐趋平缓;可培养放线菌数量先减少后增加;土壤总氮、有机碳和含水量逐渐降低,而pH值逐渐升高.C/N比率与真菌/细菌比率变化趋势相似,均为先增加后减少.土壤可培养微生物数量与理化因子的相关性分析结果表明:青藏公路沿线土壤微生物数量主要受纬度和土壤理化性质的影响,表现为微生物数量与纬度和pH值显著负相关,而与总氮、有机碳和含水量极显著正相关.
Taking the Qinghai-Tibet Highway as the research object, this paper studied the characteristics and influence factors of the quantity of soil-culturable microorganism.The results showed that the quantity of soil-culturable microorganism along the Qinghai-Tibet Highway was 0.77 × 106 ~ 2.44 × 107 CFU · g-1dw, The number of bacteria and fungi that can be cultivated in the soil decreased rapidly from the south (Shengeligong Mountain) to the north (Xidatan) and then gradually became flat. The number of culturable actinomycetes decreased at first and then increased. The total nitrogen , Organic carbon and water content decreased gradually, but pH value increased gradually.C / N ratio and fungi / bacteria ratio changed in a similar way, both increased and then decreased.The correlation analysis between quantity of soil culturable microorganism and physico-chemical factors : The number of soil microorganisms along the Qinghai-Tibet Highway is mainly affected by latitude and soil physical and chemical properties, showing that there is a significant negative correlation between the number of microorganisms and latitude and pH, and significant positive correlation with total nitrogen, organic carbon and water content.