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目的：采用反相高效液相色谱法测定吴茱萸汤不同配伍情况下的各处方中吴茱萸碱和吴茱萸次碱的含量。方法：ＯＤＳ柱，以乙腈－水－四氢呋喃－冰醋酸（４８∶５２∶１∶０１）为流动相，氯氟舒松为内标，紫外检测波长２２５ｎｍ。结果：吴茱萸单味药中吴茱萸碱和吴茱萸次碱的回收率分别为９７１％、９３７％；ＲＳＤ分别为２５％、２６％ｎ＝。结论：本方法操作简单、准确、易行。
Objective: To determine the content of evodiamine and rutacarpine in each prescription under different compatibility conditions by reversed-phase high performance liquid chromatography. Method: ODS column with acetonitrile-water-tetrahydrofuran-glacial acetic acid (48∶52:1:01) as the mobile phase, chlorofluorosulfate as the internal standard, UV detection wavelength at 225 nm. RESULTS: The recoveries of evodiamine and rutacarpine in E. rutaecarpa were 97.1% and 93.7%, respectively, and the RSDs were 2.5% and 2.6%, respectively. Conclusion: This method is simple, accurate, and easy to operate.