建立了测定全血中骨碱性磷酸酶 ( BALP)的高效液相色谱法 ( HPLC)。色谱柱为 Spectra Physics ODS反相柱 ,以 V( 0 .0 1m ol/L乙酸 ,p H 4 .8)∶ V(甲醇 ) =70∶ 30的溶液为流动相 ,流速为 0 .8m L/min,检测波长为2 75nm。当 BALP的质量浓度为 8～ 2 0 0 m g/L时 ,其活性对峰面积呈良好的线性关系 ( r=0 .996) ,检测限为 3ng。该方法简便、快速、可靠 ,可用于临床测定全血中的 BALP A high performance liquid chromatography (HPLC) method for the determination of bone alkaline phosphatase (BALP) in whole blood was established. The chromatographic column was a Spectra Physics ODS reverse phase column with a mobile phase of V (0.01 mol / L acetic acid, p H 4 .8): V (methanol) = 70: 30 with a flow rate of 0.8 m L / min, detection wavelength of 2 75nm. When the mass concentration of BALP was 8 ~ 200 mg / L, the activity of BALP showed a good linear relationship (r = 0.996) with the detection limit of 3 ng. The method is simple, rapid and reliable, and can be used clinically to determine BALP in whole blood