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AIM:To investigate the effects of growth inhibition ofhuman gastric cancer SGC-7901 cell with RRR-α-tocopherylsuccinate(VES),a derivative of natural Vitamin E,viainducing apoptosis and DNA synthesis arrest.METHODS:Human gastric cancer SGC-7901 cells wereregularly incubated in the presence of VES at 5,10 and20mg.L~(-1)(VES was dissolved in absolute ethanol anddiluted in RPMI 1640 complete condition mediacorrespondingly to a final concentration of VES and lmL.L~(-1)ethanol),succinic acid and ethanol equivalents asvehicle(VEH)control and condition-media only asuntreated(UT)control.Trypan blue dye exclusionanalysis and MTT assay were applied to detect the cellproliferation.37kBq of tritiated thymidine was added tocells and [~3H]TdR uptake was measured to observe DNAsynthesis.Apoptotic morphology was observed byelectron microscopy and DAPI staining.Flow cytometryand terminal deoxynucleotidyl transferase-mediated dUTPnick end labeling(TUNEL)assay were performed to detectVES-triggered apoptosis.RESULTS:VES inhibited SGC-7901 cell growth in a dose-dependent manner.The growth curve showed suppressionby 24.7%,49.2% and 68.7% following 24h of VEStreatment at 5,10 and 20 mg·L~(-1),respectively,similar tothe findings from MTT assay.DNA synthesis wasevidently reduced by 35%,45% and 98% after 24h VEStreatment at 20 mg·L~(-1)and 48h at 10 and 20 mg·L~(-1),respectively.VES induced SGC-7901 cells to undergoapoptosis with typically apoptotic characteristics,including morphological changes of.chromatincondensation,chromatincrescent formation/margination,nucleus fragmentation and apoptotic body formation,typical apoptotic sub-G1 peak by flow cytometry andincrease of apoptotic cells by TUNEL assay in which 90%of cells underwent apoptosis after 48h of VES treatment at20 mg·L~(-1).CONCLUSION:VES can inhibit human gastric cancer SGC-7901 cell growth by inducing apoptosis and DNA synthesisarrest,inhibition of SGC-7901 cell growth by VES is dose-and time-dependent.Therefore VES can function as apotent chemotherapeutic agent against human gastric carcinogenesis.
AIM: To investigate the effects of growth inhibition ofhuman gastric cancer SGC-7901 cells with RRR-α-tocopherylsuccinate (VES), a derivative of natural Vitamin E, viainducing apoptosis and DNA synthesis arrest. METHODS: Human gastric cancer SGC-7901 cells wereregularly incubated in the presence of VES at 5,10 and 20 mg. L -1 (VES was dissolved in absolute ethanol and duted in RPMI 1640 complete condition mediacorrespondingly to a final concentration of VES and lmL.L -1 ethanol), succinic acid and ethanol equivalents asvehicle (VEH) control and condition-media only asuntreated (UT) control. Tepan blue dye exclusionanalysis and MTT assay were applied to detect the cellproliferation. 37kBq of tritiated thymidine was added tocells and [~ 3H] TdR uptake was measured to observe DNAsynthesis. Apoptotic morphology was observed by electron microscopy and DAPI staining. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTPnick end labeling (TUNEL) assay were performed to detectVES-triggered apopto sES.RESULTS: VES inhibited SGC-7901 cell growth in a dose-dependent manner. The growth curve showed suppressionby 24.7%, 49.2% and 68.7% following 24h of VES treatment at 5, 10 and 20 mg · L -1, respectively, and similar tothe findings from the MTT assay. DNA synthesis of wasevidently reduced by 35%, 45% and 98% after 24h VEStreatment at 20 mg · L -1 and 48 h at 10 and 20 mg · L -1, respectively. VES induced SGC-7901 cells to undergoapoptosis with very apoptotic characteristics, including morphological changes of. chromatin condensation, chromatincrescent formation / margination, nucleus fragmentation and apoptotic body formation, typical apoptotic sub-G1 peak by flow cytometry and in crease of apoptotic cells by TUNEL assay in which 90% of cells underwent apoptosis after 48h of VES treatment at 20 mg · L -1. CONCLUSION: VES can inhibit human gastric cancer SGC-7901 cell growth by inducing apoptosis and DNA synthesis arrest, inhibition of SGC-7901 cell growth by VES is dose-and time-dependent.Therefore VES can funct ionas apotent chemotherapeutic agent against human gastric carcinogenesis.