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目的了解引起东莞市2011年手足口病疫情的肠道病毒型别。方法采用荧光RT-PCR方法对标本中总肠道病毒、肠道病毒71型(EV71)和柯萨奇病毒A组16型(CVA16)进行特异性核酸检测。对非EV71非CVA16的其它肠道病毒采用VP4基因测序进行型别鉴定。结果 2011年共检测608份手足口病病例标本,537份为阳性,其中198份为EV71阳性(占36.87%),128份为CVA16阳性(占23.84%),211份为非EV71、非CVA16的其它肠道病毒阳性(占39.29%)。对其它肠道病毒进行VP4基因测序分型,共获得50个可用序列,通过BLAST在线比对确定型别,1例CVA1,43例CVA6,4例CVA10,1例CVA12,1例CVB1。结论 2011年引起手足口病的非EV71非CVA16肠道病毒构成比已超过EV71和CVA16,成为东莞引起手足口病主要病原体之一,其中CVA6构成比较大,值得进一步研究。
Objective To know the type of enterovirus which caused the outbreak of HFMD in Dongguan in 2011. Methods Specific nucleic acids of total enterovirus, enterovirus 71 (EV71) and coxsackievirus A group 16 (CVA16) were detected by fluorescence RT-PCR. Other non-EV71 non-CVA16 enterovirus VP4 gene sequencing for type identification. Results A total of 608 cases of HFMD were detected in 2011, of which 537 were positive. Among them, 198 were positive for EV71 (36.87%), 128 were positive for CVA16 (23.84%), 211 were non-EV71 and non-CVA16 Other enterovirus positive (39.29%). VP4 gene sequences of other enteroviruses were sequenced and 50 available sequences were obtained. The genotypes were determined by BLAST online comparisons. One CVA1, 43 CVA6, 4 CVA10, one CVA12, and one CVB1. Conclusion The composition of non-EV71 non-CVA16 enterovirus that caused hand-foot-mouth disease in 2011 has exceeded that of EV71 and CVA16 and has become one of the major pathogens causing hand-foot-mouth disease in Dongguan. CVA6 is relatively large and deserves further study.