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目的:探讨溶血磷脂酸受体(LPA)1-3在人上皮性卵巢癌发生、发展中的可能作用。方法:采用组织微阵和免疫组化染色方法,分别对128例不同性质人卵巢肿瘤(良性、交界性、上皮性卵巢癌)及6例正常卵巢组织中LPA1-3蛋白的表达进行对比观察。结果:1LPA1蛋白在上皮性卵巢癌中的高表达率(19.48%)分别明显低于正常卵巢上皮(83.33%)、良性肿瘤(67.86%)、交界性肿瘤(58.82%),差异具有显著性(P均<0.01);上皮性卵巢癌LPA2和LPA3蛋白高表达率(59.74%,64.94%)则分别明显高于正常卵巢上皮(16.67%,16.67%)、良性肿瘤(17.86%,28.57%)和交界性肿瘤(29.41%,35.29%),差异具有统计学意义(P<0.05或0.01)。2在77例上皮性卵巢癌中,LPA1、LPA2和LPA3蛋白的高表达率在不同组织学类型(浆液性、黏液性、子宫内膜样及透明细胞性)及不同分化程度(高、中分化和低分化)的上皮性卵巢癌中均无明显差异(P均>0.05)。3LPA1蛋白高表达率在复发性病例(5.88%)和非复发性病例(23.33%)中的表达无明显差异(P>0.05),但LPA2和LPA3蛋白在复发性卵巢癌的高表达率(82.35%,82.35%)分别明显高于非复发性卵巢癌病例(53.33%,55.00%)(P均<0.05)。结论:LPA1蛋白在卵巢癌发生中的作用不明显,而LPA2和LPA3蛋白的高表达与上皮性卵巢癌的发生和复发明显相关。
Objective: To investigate the possible role of lysophosphatidic acid receptor (LPA) 1-3 in the occurrence and development of human epithelial ovarian cancer. Methods: The expression of LPA1-3 protein in 128 cases of different types of human ovarian tumors (benign, borderline, epithelial ovarian cancer) and 6 cases of normal ovarian tissue were observed by tissue microarray and immunohistochemistry. Results: The high expression rate of 1LPA1 protein in epithelial ovarian cancer (19.48%) was significantly lower than that in normal ovarian epithelium (83.33%), benign tumor (67.86%) and borderline tumor (58.82%), respectively P <0.01). The high expression rates of LPA2 and LPA3 in epithelial ovarian cancer (59.74%, 64.94%) were significantly higher than those in normal epithelium (16.67%, 16.67%), benign tumors (17.86%, 28.57% Borderline tumors (29.41%, 35.29%), the difference was statistically significant (P <0.05 or 0.01). 2 In 77 cases of epithelial ovarian cancer, the high expression rates of LPA1, LPA2 and LPA3 protein were significantly different in different histological types (serous, mucinous, endometrioid and clear cell) and in different degree of differentiation And poorly differentiated) epithelial ovarian cancer (P> 0.05). There was no significant difference in the expression of 3LPA1 between recurrent cases (5.88%) and non-recurrent cases (23.33%) (P> 0.05), but the high expression rates of LPA2 and LPA3 in recurrent ovarian cancer (82.35 %, 82.35%) were significantly higher than those in non - recurrent ovarian cancer (53.33%, 55.00%, respectively) (all P <0.05). Conclusion: The role of LPA1 protein in ovarian cancer is not obvious. However, the high expression of LPA2 and LPA3 proteins is significantly associated with the occurrence and recurrence of epithelial ovarian cancer.