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实验制备多孔Si材料作为传感器载体,通过H2O2浸泡的方法使其表面形成Si-OH键,然后对样品进行硅烷化处理。用戊二醛作为偶联剂,通过共价键偶联法将血管紧张素-Ⅱ(Ang-Ⅱ)抗体固定到多孔Si的孔洞中。分别滴加不同浓度的抗原使其发生反应,测量傅里叶变换红外谱(FT-IR)和加入抗体前后反射谱的变化,结果表明,硅烷化效果明显,生物抗原较好固定,抗原抗体反应前后反射谱有明显红移,且红移量于滴加抗原浓度增加而增加,并呈现抗原抗体浓度于红移量的非线性关系。
Experimental preparation of porous Si material as a sensor carrier, soaked by H2O2 to form Si-OH bond on the surface, and then the sample was silanized. Angiotensin II (Ang-II) antibody was immobilized to the pores of porous Si by covalent coupling using glutaraldehyde as a coupling agent. Different concentrations of antigens were added dropwise to make them react. The changes of FT-IR and reflectance before and after the addition of antibody were measured. The results showed that the silanization effect was obvious, the biologic antigens were well fixed and the antigen-antibody reaction There was a significant redshift of the reflectance spectra before and after, and the amount of redshift increased with the dropping of antigen concentration, showing a nonlinear relationship between the concentration of antigen and antibody and the amount of red shift.