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本研究采用墨西哥利什曼原虫亚马孙亚种(L.m.a.)及巴西利什曼原虫巴拿马亚种(L.b.p.),分别感染叙利亚金色仓鼠及非洲白尾鼠;再将吸食这两种鼠血的白蛉分别制戒匀浆,接种于血琼脂平板,以检测白蛉吸入的虫数及其发育情况。作者在仓鼠后足垫及白尾鼠尾根部分别皮内注射L.m.a.及L.b.p.前鞭毛体各1.5×10~7个。3～7个月后,局部产生病变。麻醉病鼠,让长须白蛉吸食病变部血。取鼠病变组织浓集无鞭毛体,将含5.25或50个L.m.a.或L.b.p.无鞭毛体的50μl悬液,分别
In this study, Leishmania mexicana (Lma) and Leishmania braziliensis (Lbp) were used to infect Syrian golden hamster and African white-haired rat, respectively. Ligation homogenate, inoculation of blood agar plate to detect the number of white sand inhalation and its development. The authors injected intramuscular injection of L.m.a. and L.b.p. promastigotes 1.5 × 10 ~ 7 each in the hamster hind footpad and tail of the white tail. 3 to 7 months later, local lesions. Anesthetize rats, so long mustache bait lesion blood. Take mice lesion tissue concentration of amastigotes, will contain 5.25 or 50 L.m.a. or L.b.p. flagella 50μl suspension, respectively