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用乳酸钠作为丙酮酸的前体物质,生物合成L-苯基乙酰基甲醇(L-Phenylacetylcarbinol,L-PAC),控制NADH来抑制醇脱氢酶的活性降低副反应,提高酵母活细胞生物合成的L-PAC产量。实验结果表明,乳酸钠合成L-PAC的最佳反应条件为时间8 h,温度30℃,pH6.5,乳酸钠用量是22 mmol/L,L-PAC产量为12.3g/L。生产等量L-PAC所耗的丙酮酸为160 mmol/L,采用乳酸钠作为丙酮酸的替代品,可大幅度降低生产成本,并有利于工业化生产。
L-Phenylacetylcarbinol (L-PAC) is biosynthesized by using sodium lactate as the precursor of pyruvic acid, controlling NADH to inhibit the activity of alcohol dehydrogenase to reduce the side reaction and improve the biosynthesis of yeast living cells L-PAC production. The experimental results showed that the optimal reaction conditions for the synthesis of L-PAC were as follows: time 8 h, temperature 30 ℃, pH 6.5, sodium lactate 22 mmol / L and L-PAC 12.3 g / L. L-PAC production equivalent amount of pyruvate consumed 160 mmol / L, the use of sodium lactate as a substitute for pyruvate, can significantly reduce production costs, and is conducive to industrial production.